Skip to main content
Log in

Extraction, molecular fractionation and enzyme degradation of organically associated phosphorus in soil solutions

  • Original Paper
  • Published:
Biology and Fertility of Soils Aims and scope Submit manuscript

Abstract

The concentrations and chemical composition of water-extractable P were compared in four soil types from NE Scotland. All sites were sampled during the early establishment phase of a spring barley (Hordeum vulgare) crop. The quantity of total soluble P extracted ranged from <2.0 to 10 mg P kg soil-1, of which up to 50% was classified as being organically associated. Sample fractionation showed that both orthophosphate inorganic P and organic P were associated with a wide molecular-size range of organic material. A strong positive correlation was readily apparent between P and the sum of Fe + Al in the fractionated samples. The extent of enzymatic hydrolysis of organic P varied between soil samples and the type of enzyme. Phytase consistently produced the greatest degree of hydrolysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson G (1980) Assessing organic phosphorus in soils. Khasawneh FE, Sample EC, Kamprath EJ (eds) The role of phosphorus in agriculture. Am Soc Agron, Madison, Wis, pp 411–431

    Google Scholar 

  • Brooks PC, Powlson DS, Jenkinson DS (1984) Phosphorus in the soil microbial biomass. Soil Biol Biochem 16:169–175

    Google Scholar 

  • Gerke J (1992) Orthophosphate and organic phosphate in the soil solution of four sandy soils in relation to pH: Evidence for humic-Fe-(Al-) phosphate complexes. Commun Soil Sci Plant Anal 23:601–612

    Google Scholar 

  • Gerke J, Jungk A (1991) Separation of phosphorus bound to organic matrices from inorganic phosphorus in alkaline soil extracts by ultrafiltration. Commun Soil Sci Plant Anal 22: 1621–1630

    Google Scholar 

  • Harrison AF (1987) Soil organic phosphorus. A review of world literature. CAB International, Wallingford

    Google Scholar 

  • Herbes SE, Allex HE, Mancy KH (1975) Enzymatic characterisation of soluble organic phosphorus in lake water. Science 187:432–434

    Google Scholar 

  • Islam A, Mandal R, Osman KT (1979) Direct absorption of organic phosphate by rice and jute plants. Plant and Soil 53:49–54

    Google Scholar 

  • Jones DL, Darrah PR (1992) Re-sorption of organic components by roots of Zea mays L. and its consequences in the rhizosphere. I. Re-sorption of 14C labelled glucose, mannose and citric acid. Plant and Soil 143:259–266

    Google Scholar 

  • Jones RI, Salonen K, De Haan H (1988) Phosphorus transformations in the epilimnion of humic lakes: Abiotic interactions between dissolved humic materials and phosphate. Freshwater Biol 19:357–369

    Google Scholar 

  • Levesque M (1969) Characterisation of model and soil organic matter-metal complexes. Can J Soil Sci 49:365–373

    Google Scholar 

  • Malcolm RE, Vaughan D (1979) Comparative effects of soil organic matter fractions on phosphatase activities in wheat roots. Plant and Soil 51:117–126

    Google Scholar 

  • Macaulay Institute for Soil Research and Scottish Agricultural Colleges (1985) Advisory soil analysis and interpretation. MISR/SAC, Bull 1

  • Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chem Acta 27:31–36

    Google Scholar 

  • Moyer JR, Thomas RL (1970) Organic phosphorus and inositol phosphates in molecular size fractions of a soil organic matter extract. Soil Sci Soc Am Proc 34:80–83

    Google Scholar 

  • Reith JWS, Inkson RHE, Scott NM, Caldwell KS, Ross JAM, Simpson WE (1987) Estimates of soil phosphorus for different soil series. Fert Res 11:123–142

    Google Scholar 

  • Ron Vaz MD, Edwards AC, Shand CA, Cresser MS (1993) Phosphorus fractions in soil solution: Influence of soil acidity and fertiliser additions. Plant and Soil 148:175–183

    Google Scholar 

  • Stainton MP (1980) Errors in the molybdenum blue methods for determining ortho-phosphate in fresh water. Can J Fish Aqua Sci 37:472–478

    Google Scholar 

  • Tate KR (1985) Soil phosphorus. In: Vaughan D, Malcolm RE (eds) Soil organic matter and biological activity. Martinus Nijhoff/Dr W Junk, Dordrecht, pp 329–377

    Google Scholar 

  • Tarafdar JC, Claassen N (1988) Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatase produced by plant roots and microorganisms. Biol Fertil Soils 5:308–312

    Google Scholar 

  • Thomas RL, Bowman BT (1966) The occurrence of high molecular weight organic phosphorus compounds in soil. Soil Sci Soc Am Proc 30:799–801

    Google Scholar 

  • White RE, Thomas GW (1981) Hydrolysis of aluminium on weakly acidic organic exchangers: Implications for phosphate adsorption. Fert Res 2:159–167

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pant, H.K., Edwards, A.C. & Vaughan, D. Extraction, molecular fractionation and enzyme degradation of organically associated phosphorus in soil solutions. Biol Fertil Soils 17, 196–200 (1994). https://doi.org/10.1007/BF00336322

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00336322

Key words

Navigation