Bacterial, fungal and protozoan responses to chloroform fumigation in stored soil
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2021, City and Environment InteractionsCitation Excerpt :The soil was sieved (2 mm), and active bacterial and fungal-hyphae biomasses were measured using fluorescein diacetate staining. The length of active and total fungal hyphae was determined following the procedures of Ingham and Klein [42], Ingham and Horton [43], and Lodge and Ingham [56]. The biovolume-to-biomass conversion rates of Van Veen and Paul [85] were applied as necessary.
Separating cellular metabolism from exoenzyme activity in soil organic matter decomposition
2014, Soil Biology and BiochemistryCitation Excerpt :The fumigation itself typically lasts 1–3 days (Jenkinson and Powlson, 1976; Foster, 1988; Dickens and Anderson, 1999). If given enough time, CHCl3 diffuses into soil micropores and kills most—but not all—soil bacteria and fungi (Ingham and Horton, 1987; Alphei and Scheu, 1993; Toyota et al., 1996). Soils may also be incubated under CHCl3 to prevent microbial regrowth.
Comments on the paper by Kemmitt et al. (2008) 'Mineralization of native soil organic matter is not regulated by the size, activity or composition of the soil microbial biomass - A new perspective' [Soil Biology & Biochemistry 40, 61-73]: The biology of the Regulatory Gate
2009, Soil Biology and BiochemistryQuantitative assessment of the fungal contribution to microbial tissue in soil
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2006, Forest Ecology and Management
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Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, U.S.A.
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Department of Plant and Soil Biology, University of California, Berkeley, CA 94720, U.S.A.