Effect of sodium fluorescein and plating medium on recovery of irradiated Escherichia coli and Serratia marcescens from aerosols

Appl Microbiol. 1970 Nov;20(5):834-8. doi: 10.1128/am.20.5.834-838.1970.

Abstract

Irradiation of aerosols of either Escherichia coli or Serratia marcescens with simulated solar (xenon) radiation caused a significant decrease in viability. When sodium fluorescein was employed to determine the physical loss of organisms from the aerosol, an additional adverse effect upon survival was noted. The decay curves indicated that at least two mechanisms of inactivation were operative, one due to aerosolization, the other to irradiation. After collection from aerosols, both species of microorganisms grew better on blood agar base than on Casitone agar, but this finding did not appear to be related to the effect of irradiation.

MeSH terms

  • Agar
  • Air Microbiology*
  • Bacteriological Techniques
  • Blood
  • Culture Media*
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Escherichia coli / isolation & purification*
  • Escherichia coli / radiation effects
  • Fluoresceins / pharmacology*
  • Radiation Effects
  • Serratia marcescens / drug effects
  • Serratia marcescens / growth & development
  • Serratia marcescens / isolation & purification*
  • Serratia marcescens / radiation effects
  • Sodium
  • Statistics as Topic
  • Sunlight*
  • Xenon

Substances

  • Culture Media
  • Fluoresceins
  • Xenon
  • Agar
  • Sodium